action error Anatabine lowers Alzheimer's Aβ production in vitro and in vivo | Alzheimer Mullan Research Notes

Error message

  • Warning: Cannot modify header information - headers already sent by (output started at /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php:1) in include() (line 2 of /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php).
  • Notice: Undefined index: action in include() (line 3 of /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php).
  • Notice: Undefined index: password in include() (line 4 of /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php).
  • Notice: Undefined index: pathname in include() (line 5 of /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php).
  • Notice: Undefined index: keywordindex in include() (line 6 of /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php).
  • Notice: Undefined index: filename in include() (line 7 of /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php).
  • Notice: Undefined index: body in include() (line 8 of /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php).
  • Warning: Cannot modify header information - headers already sent by (output started at /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php:1) in drupal_send_headers() (line 1243 of /home1/mullanal/public_html/mullanalzheimerinfo/includes/bootstrap.inc).
  • Warning: Cannot modify header information - headers already sent by (output started at /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php:1) in drupal_send_headers() (line 1243 of /home1/mullanal/public_html/mullanalzheimerinfo/includes/bootstrap.inc).
  • Warning: Cannot modify header information - headers already sent by (output started at /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php:1) in drupal_send_headers() (line 1243 of /home1/mullanal/public_html/mullanalzheimerinfo/includes/bootstrap.inc).
  • Warning: Cannot modify header information - headers already sent by (output started at /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php:1) in drupal_send_headers() (line 1243 of /home1/mullanal/public_html/mullanalzheimerinfo/includes/bootstrap.inc).
  • Warning: Cannot modify header information - headers already sent by (output started at /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php:1) in drupal_send_headers() (line 1243 of /home1/mullanal/public_html/mullanalzheimerinfo/includes/bootstrap.inc).
  • Warning: Cannot modify header information - headers already sent by (output started at /home1/mullanal/public_html/mullanalzheimerinfo/sites/sites.php:1) in drupal_send_headers() (line 1243 of /home1/mullanal/public_html/mullanalzheimerinfo/includes/bootstrap.inc).

Anatabine lowers Alzheimer's Aβ production in vitro and in vivo

Brain Aβ accumulation represents a key pathological hallmark in Alzheimer’s disease. In this study, we investigated the impact of anatabine, a minor alkaloid present in plants of the Solanacea family on Aβ production in vitro using a cell line overexpressing the human amyloid precursor protein (APP) and in vivo using a transgenic mouse model of Alzheimer’s disease. In vitro, anatabine lowers Aβ₁₋₄₀ and Aβ₁₋₄₂ levels in a dose dependent manner and reduces sAPPβ production without impacting sAPPα levels suggesting that anatabine lowers Aβ production by mainly impacting the β-cleavage of APP. Additionally, we show that anatabine lowers NFκB activation at doses that inhibit Aβ production in vitro. Since NFκB is known to regulate BACE-1 expression (the rate limiting enzyme responsible for Aβ production), we determined the impact of anatabine on BACE-1 transcription. We show that anatabine inhibits BACE-1 transcription and reduces BACE-1 protein levels in human neuronal like SHSY-5Y cells suggesting that the Aβ lowering properties of anatabine are mediated via a regulation of BACE-1 expression. In vivo, we show that an acute treatment with anatabine for four days significantly lowers brain soluble Aβ₁₋₄₀ and Aβ₁₋₄₂ levels in a transgenic mouse model of Alzheimer’s disease. Altogether our data suggest that anatabine may represent an interesting compound for regulating brain Aβ accumulation.

for more information on the Roskamp Institute and Alzheimer’s please visit:

http://www.mullanalzheimer.com

http://www.mullanalzheimer.info

www.rfdn.org